plx trc311 vector (Addgene inc)
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Addgene inc
plx trc311 vector
Plx Trc311 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plx+trc311/pCDB325+(Plasmid+%23113669)/pm41588195-309-1-3
Average 94 stars, based on 1 article reviews
Plx Trc311 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plx+trc311/pCDB325+(Plasmid+%23113669)/pm41588195-309-1-3
Average 94 stars, based on 1 article reviews
plx trc311 vector - by Bioz Stars,
2026-11
94/100 stars
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Construct:Article Title: The CD58:CD2 axis is co-regulated with PD-L1 via CMTM6 and governs anti-tumor immunity Article Snippet: CD58, CMTM6, PD-L1, and PD-L1 H1Amut open reading frames (ORFs) with a 5’ Kozak sequence were flanked by att B cloning sites and ordered as gene blocks (GeneWiz), which were then cloned into pDONRTM221 (Thermo) to create an entry clone using the Gateway Cloning system (Thermo). .. Donor constructs were then cloned into the constitutive expression vector pLEX_307 (gift from David Root; Addgene #41392) or Clone Assay:Article Title: The CD58:CD2 axis is co-regulated with PD-L1 via CMTM6 and governs anti-tumor immunity Article Snippet: CD58, CMTM6, PD-L1, and PD-L1 H1Amut open reading frames (ORFs) with a 5’ Kozak sequence were flanked by att B cloning sites and ordered as gene blocks (GeneWiz), which were then cloned into pDONRTM221 (Thermo) to create an entry clone using the Gateway Cloning system (Thermo). .. Donor constructs were then cloned into the constitutive expression vector pLEX_307 (gift from David Root; Addgene #41392) or Expressing:Article Title: The CD58:CD2 axis is co-regulated with PD-L1 via CMTM6 and governs anti-tumor immunity Article Snippet: CD58, CMTM6, PD-L1, and PD-L1 H1Amut open reading frames (ORFs) with a 5’ Kozak sequence were flanked by att B cloning sites and ordered as gene blocks (GeneWiz), which were then cloned into pDONRTM221 (Thermo) to create an entry clone using the Gateway Cloning system (Thermo). .. Donor constructs were then cloned into the constitutive expression vector pLEX_307 (gift from David Root; Addgene #41392) or Article Title: Loss of Ataxin-1 Potentiates Alzheimer's Pathogenesis by Elevating Cerebral BACE1 Transcription Article Snippet: The PCR reactions were performed at least duplicate for each sample and the average CT values were used to calculate the mRNA expression levels (CFX96 Touch Real-Time PCR Detection System, Bio-Rad). .. One day after seeding, the cells were transfected with 100 ng pGL3-BACE1 promoter, 50 ng pRL-TK (for renilla luciferase expression, Promega), and either 200 ng of Article Title: A Microtubule Mechanostat Enables Cells to Navigate Confined Environments Article Snippet: All entry plasmids were propagated in XL2-blue ultracompetent E. coli (200150, Agilent) and sequence verified via sanger sequencing (Australian Genome Research Facility, St Lucia). .. Lentiviral expression vectors were created by Gateway Gene Cloning (Invitrogen, 11791-020) with Gateway LR Clonase II (Invitrogen, 11791) into either of the following destination vectors: Plasmid Preparation:Article Title: The CD58:CD2 axis is co-regulated with PD-L1 via CMTM6 and governs anti-tumor immunity Article Snippet: CD58, CMTM6, PD-L1, and PD-L1 H1Amut open reading frames (ORFs) with a 5’ Kozak sequence were flanked by att B cloning sites and ordered as gene blocks (GeneWiz), which were then cloned into pDONRTM221 (Thermo) to create an entry clone using the Gateway Cloning system (Thermo). .. Donor constructs were then cloned into the constitutive expression vector pLEX_307 (gift from David Root; Addgene #41392) or Article Title: Acetyl-Coenzyme A Synthetase 2 Potentiates Macropinocytosis and Muscle Wasting Through Metabolic Reprogramming in Pancreatic Cancer. Article Snippet: All cell lines have been authenticated and evaluated as mycoplasma free. hACSS2 plasmid (EX-Z9293-Lv217) and the empty control vector (EX-NEG-Lv217) were purchased from GeneCopoeia (Rockville, MD). .. The eSpCas9(1.1) (Plasmid #71814), lenti single guide RNA (MS2)_puro optimized backbone (Plasmid #73797), Transfection:Article Title: Loss of Ataxin-1 Potentiates Alzheimer's Pathogenesis by Elevating Cerebral BACE1 Transcription Article Snippet: The PCR reactions were performed at least duplicate for each sample and the average CT values were used to calculate the mRNA expression levels (CFX96 Touch Real-Time PCR Detection System, Bio-Rad). .. One day after seeding, the cells were transfected with 100 ng pGL3-BACE1 promoter, 50 ng pRL-TK (for renilla luciferase expression, Promega), and either 200 ng of Luciferase:Article Title: Loss of Ataxin-1 Potentiates Alzheimer's Pathogenesis by Elevating Cerebral BACE1 Transcription Article Snippet: The PCR reactions were performed at least duplicate for each sample and the average CT values were used to calculate the mRNA expression levels (CFX96 Touch Real-Time PCR Detection System, Bio-Rad). .. One day after seeding, the cells were transfected with 100 ng pGL3-BACE1 promoter, 50 ng pRL-TK (for renilla luciferase expression, Promega), and either 200 ng of Cloning:Article Title: A Microtubule Mechanostat Enables Cells to Navigate Confined Environments Article Snippet: All entry plasmids were propagated in XL2-blue ultracompetent E. coli (200150, Agilent) and sequence verified via sanger sequencing (Australian Genome Research Facility, St Lucia). .. Lentiviral expression vectors were created by Gateway Gene Cloning (Invitrogen, 11791-020) with Gateway LR Clonase II (Invitrogen, 11791) into either of the following destination vectors: other:Article Title: Loss of Ataxin-1 Potentiates Alzheimer's Pathogenesis by Elevating Cerebral BACE1 Transcription Article Snippet: DAPI (4’,6-diamidino-2-phenylindole, Sigma) was used for nuclear counter staining, and DABCO (1,4-diazabicyclo[2.2.2]octane, Sigma) was used for mounting. Article Title: The CD58-CD2 axis is co-regulated with PD-L1 via CMTM6 and shapes anti-tumor immunity. Article Snippet: Article |
Fig. S4A ). (B) UMAP plot of cells from control (left) and Etv1 mutant (right) organoids (control, 494 cells; Etv1 mutant, 664 cells). (C) Percentage of cells found in each of the identified cell clusters in control (two lines) and Etv1 mutant (three lines) organoids. Error bars indicate s.d. Significance was evaluated with an unpaired two-tailed t -test. ns, not significant. (D) Violin plots showing expression levels of known cell type and proliferation marker genes in control (orange) and Etv1 mutant (green) organoids. (E) UMAP plot showing EECs in control (orange) and Etv1 mutant (green) organoids. (F) UMAP plot showing expression levels of Etv1 , Gcg , Cck and Pyy in EECs from control (top row) and Etv1 mutant (bottom row) organoids. " width="100%" height="100%">
Fig. S7A ). Luciferase activity was normalised to the activity in HEK293 cells transfected with a pGL4.23 vector without any insert. Where indicated, cells were treated for 24 h with doxycycline (1 mg/ml). Error bars indicate s.d. ( n =4). Significance was evaluated with an unpaired two-tailed t -test. ns, not significant; * P <0.05, ** P <0.01. " width="100%" height="100%">